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esp3i restriction site  (New England Biolabs)


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    Structured Review

    New England Biolabs esp3i restriction site
    Esp3i Restriction Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 538 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/esp3i+restriction+sites/Esp3I/pm40804173-244-1-21
    Average 97 stars, based on 538 article reviews
    esp3i restriction site - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage
    Article Snippet: .. All sgRNA sequences are shown (Table S1), and were cloned into the pCF120 and pCF123 vector using BbsI restriction sites and enzymes (New England Biolabs) or into the pCF221 vector using Esp3I restriction sites and enzymes (New England Biolabs). ..

    Article Title: Targeting the non-coding genome and temozolomide signature enables CRISPR-mediated glioma oncolysis.
    Article Snippet: .. All sgRNA sequences were cloned into the pCF820, pCF821, pCF826, and pCF827 vectors using Esp3I restriction sites and enzymes (New England Biolabs). .. Viral transduction and generation of Cas9-expressing cell lines Generally, lentiviral particles were produced in HEK293T cells or derivatives thereof using polyethylenimine (PEI; Polysciences, #23966) mediated transfection of plasmids, as previously described.45 In brief, lentiviral transfer vectors were co-transfected with the lentiviral helper plasmid psPAX2 (a gift from Didier Trono; Addgene, #12260) and the VSV-G envelope plasmid pMD2.G (a gift from Didier Trono; Addgene, #12259).

    Article Title: Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage
    Article Snippet: .. All sgRNA sequences are shown (Table S1), and were cloned into the pCF120 and pCF123 vector using BbsI restriction sites and enzymes (New England Biolabs) or into the pCF221 vector using Esp3I restriction sites and enzymes (New England Biolabs). ..

    Article Title: Targeting the non-coding genome and temozolomide signature enables CRISPR-mediated glioma oncolysis
    Article Snippet: .. All sgRNA sequences were cloned into the pCF820, pCF821, pCF826, and pCF827 vectors using Esp3I restriction sites and enzymes (New England Biolabs). .. Generally, lentiviral particles were produced in HEK293T cells or derivatives thereof using polyethylenimine (PEI; Polysciences, #23966) mediated transfection of plasmids, as previously described.

    Polymerase Chain Reaction:

    Article Title: Shelf-Life Extension of Fc-Fused Single Chain Fragment Variable Antibodies by Lyophilization
    Article Snippet: Cloning was performed via Golden gate assembly approach ( ) with Esp3I endonuclease (New England Biolabs) and T4 DNA ligase (Promega). .. For this purpose, the Esp3I restriction sites were introduced into the desired VH and VL by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs). ..

    Agarose Gel Electrophoresis:

    Article Title: Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries
    Article Snippet: .. After generating the substrate mixture with NNK degenerated reverse primers, the fragments containing cDNA mixture were then extracted from agarose gel, inserted into pSin-ELYK template vector between Esp3I restriction sites through the Golden Gate assembly (NEB) to generate the biosensor library. .. The product was purified and concentrated by using DNA Clean and Concentrator Kits (Zymo Research) and transformed into ElectroMAXTM DH10BTM Cells (Invitrogen, Catalog number: 18290015), which are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 × 10 10 CFU/μg plasmid DNA.

    Article Title: Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries.
    Article Snippet: .. After generating the substrate mixture with NNK degenerated reverse primers, the fragments containing cDNA mixture were then extracted from agarose gel, inserted into pSin-ELYK template vector between Esp3I restriction sites through the Golden Gate assembly (NEB) to generate the biosensor library. .. The product was purified and concentrated by using DNA Clean and Concentrator Kits (Zymo Research) and transformed into ElectroMAXTM DH10BTM Cells (Invitrogen, Catalog number: 18290015), which are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 × 1010 CFU/μg plasmid DNA.



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